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donkey anti goat igg h l  (Bio-Rad)


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    Structured Review

    Bio-Rad donkey anti goat igg h l
    Donkey Anti Goat Igg H L, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+goat+igg/Donkey+anti+Goat+IgG+(H%2FL)+(Rat%2FMouse+Adsorbed)/bio_rxiv__64898__2026__04__27__720799-52-17-15
    Average 93 stars, based on 31 article reviews
    donkey anti goat igg h l - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Coenzyme Q10 Inhibits Glutamate Excitotoxicity and Oxidative Stress–Mediated Mitochondrial Alteration in a Mouse Model of Glaucoma
    Article Snippet: The primary antibodies were mouse monoclonal anti-GFAP antibody (1:3000; Sigma-Aldrich Corp., St. Louis, MO), rabbit polyclonal anti– N -methyl- d -aspartate receptor (NR) 1 antibody (1:1000; BD Pharmingen, San Diego, CA), rabbit polyclonal anti-NR2A antibody (1:500; Millipore, Billerica, MA), rabbit polyclonal anti–superoxide dismutase-2 (SOD2) antibody (1:5000; Santa Cruz Biotechnology), rabbit polyclonal anti–heme oxygenase-1 (HO1) antibody (1:1000; Millipore), mouse monoclonal anti-actin antibody (1:5000; Millipore), rabbit polyclonal anti-Tfam antibody (1:3000; Santa Cruz Biotechnology), mouse monoclonal anti–total OXPHOS complex antibody (1:3000; Invitrogen), rabbit polyclonal anti-Porin antibody (1:1000; Calbiochem, La Jolla, CA), rabbit polyclonal anti-Bax antibody (1:500; Santa Cruz Biotechnology), and rabbit polyclonal anti–phosphorylated Bad (pBad) antibody (1:1000; Cell Signaling, Danvers, MA). .. After several washes in PBST, the membranes were incubated with peroxidase-conjugated goat anti-mouse IgG (1:5000; Bio-Rad, Hercules, CA), goat anti-rabbit IgG (1:5000; Bio-Rad), or donkey anti-goat IgG (1:5000; Bio-Rad) and developed by film using chemiluminescence detection (ECL Plus; GE Healthcare Bioscience, Piscataway, NJ). ..

    Article Title: Neuroepithelial stem cell marker nestin regulates the migration, invasion and growth of human gliomas.
    Article Snippet: .. The membranes were washed and incubated with HRP-labeled donkey anti-goat IgG (dilution, 1:4,000) for 60 min. After washing, the blots were visualized using enhanced chemiluminescence and detected using a ChemiDoc XRS system (Bio-Rad Laboratories, Inc., Hercules, CA). ..

    Article Title: The effect of 17β-estradiol on sex-dimorphic cytochrome P450 expression patterns induced by hyperoxia in the liver of male CBA/H mice.
    Article Snippet: After sonification (3 9 30 s), whole liver homogenates were centrifuged at 16.0009g for 20 min at ?4 C. Supernatant was collected and total cellular proteins (75 lg per lane) were resolved by denaturing SDS-PAGE, and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). .. Membranes were blocked in 5 % nonfat dry milk in TN buffer (50 mM TRIS, 150 mM NaCl, pH = 7.4) overnight at ?4 C. Membranes were incubated with primary polyclonal goat antibody against Cyp7B1 (Santa Cruz Biotechnology Inc, TX, USA) (diluted 1:200 and incubated overnight at ?4 C), followed by incubation with donkey anti-goat IgG, horseradish peroxidase-conjugated secondary antibody (BioRad, Hercules, CA, USA) for 1 h at room temperature. .. For Cyp2E1 protein detection, membranes were incubated with primary polyclonal rabbit antibody against Cyp2E1 (Abcam, Cambridge, UK), diluted 1:200 and incubated overnight at ?4 C, followed by incubation with donkey anti-rabbit IgG, horseradish peroxidase-conjugated, secondary antibody (Amersham Biosciences Inc., USA) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Primary fatty acid amide metabolism: conversion of fatty acids and an ethanolamine in N 18 TG 2 and SCP cells
    Article Snippet: The PCR product was gel purified using a QIAQuick gel extraction kit (Qiagen), and the product was sequenced to verify accuracy. .. Linoleic acid, palmitic acid, 6-thioguanine, and fatty acid-free BSA (BSA) were from Sigma; elaidic acid and palmitoleic acid were from Fisher; penicillin/streptomycin, EMEM, and DMEM were supplied by Mediatech Cellgro; fetal bovine serum (FBS) was from Atlanta Biologicals; BSTFA and silica were from Suppelco; [ 2 H 33 ]heptadecanoic acid (margaric acid, C 2 H 3 -(C 2 H 2 ) 15 -COOH) was from C/D/N isotopes; goat ant-FAAH (V-17) antibody and its blocking peptide were from Santa Cruz Biotech; donkey anti-goat IgG fused to horse radish peroxidase (HRP) was from ICN Biomedical; SuperSignal chemiluminescent HRP substrate was from Bio-Rad; and MagicMark XP molecular weight markers were from Invitrogen. .. Mouse neuroblastoma N 18 TG 2 cells were purchased from the Deutsche Sammlung von Mikrooganism und Zellkuturen GmBH, and the sheep choroid plexus cells came from the American Type Culture Collection.

    Article Title: Primary fatty acid amide metabolism: conversion of fatty acids and an ethanolamine in N 18 TG 2 and SCP cells
    Article Snippet: .. Materials and chemicals Linoleic acid, palmitic acid, 6-thioguanine, and fatty acid-free BSA (BSA) were from Sigma; elaidic acid and palmitoleic acid were from Fisher; penicillin/streptomycin, EMEM, and DMEM were supplied by Mediatech Cellgro; fetal bovine serum (FBS) was from Atlanta Biologicals; BSTFA and silica were from Suppelco; [ 2 H 33 ]heptadecanoic acid (margaric acid, C 2 H 3 -(C 2 H 2 ) 15 -COOH) was from C/D/N isotopes; goat ant-FAAH (V-17) antibody and its blocking peptide were from Santa Cruz Biotech; donkey anti-goat IgG fused to horse radish peroxidase (HRP) was from ICN Biomedical; SuperSignal chemiluminescent HRP substrate was from Bio-Rad; and MagicMark XP molecular weight markers were from Invitrogen. .. Mouse neuroblastoma N 18 TG 2 cells were purchased from the Deutsche Sammlung von Mikrooganism und Zellkuturen GmBH, and the sheep choroid plexus cells came from the American Type Culture Collection.

    Molecular Weight:

    Article Title: Primary fatty acid amide metabolism: conversion of fatty acids and an ethanolamine in N 18 TG 2 and SCP cells
    Article Snippet: The PCR product was gel purified using a QIAQuick gel extraction kit (Qiagen), and the product was sequenced to verify accuracy. .. Linoleic acid, palmitic acid, 6-thioguanine, and fatty acid-free BSA (BSA) were from Sigma; elaidic acid and palmitoleic acid were from Fisher; penicillin/streptomycin, EMEM, and DMEM were supplied by Mediatech Cellgro; fetal bovine serum (FBS) was from Atlanta Biologicals; BSTFA and silica were from Suppelco; [ 2 H 33 ]heptadecanoic acid (margaric acid, C 2 H 3 -(C 2 H 2 ) 15 -COOH) was from C/D/N isotopes; goat ant-FAAH (V-17) antibody and its blocking peptide were from Santa Cruz Biotech; donkey anti-goat IgG fused to horse radish peroxidase (HRP) was from ICN Biomedical; SuperSignal chemiluminescent HRP substrate was from Bio-Rad; and MagicMark XP molecular weight markers were from Invitrogen. .. Mouse neuroblastoma N 18 TG 2 cells were purchased from the Deutsche Sammlung von Mikrooganism und Zellkuturen GmBH, and the sheep choroid plexus cells came from the American Type Culture Collection.

    Article Title: Primary fatty acid amide metabolism: conversion of fatty acids and an ethanolamine in N 18 TG 2 and SCP cells
    Article Snippet: .. Materials and chemicals Linoleic acid, palmitic acid, 6-thioguanine, and fatty acid-free BSA (BSA) were from Sigma; elaidic acid and palmitoleic acid were from Fisher; penicillin/streptomycin, EMEM, and DMEM were supplied by Mediatech Cellgro; fetal bovine serum (FBS) was from Atlanta Biologicals; BSTFA and silica were from Suppelco; [ 2 H 33 ]heptadecanoic acid (margaric acid, C 2 H 3 -(C 2 H 2 ) 15 -COOH) was from C/D/N isotopes; goat ant-FAAH (V-17) antibody and its blocking peptide were from Santa Cruz Biotech; donkey anti-goat IgG fused to horse radish peroxidase (HRP) was from ICN Biomedical; SuperSignal chemiluminescent HRP substrate was from Bio-Rad; and MagicMark XP molecular weight markers were from Invitrogen. .. Mouse neuroblastoma N 18 TG 2 cells were purchased from the Deutsche Sammlung von Mikrooganism und Zellkuturen GmBH, and the sheep choroid plexus cells came from the American Type Culture Collection.

    other:

    Article Title: Antithrombin-binding heparan sulfate is ubiquitously expressed in epithelial cells and suppresses pancreatic tumorigenesis
    Article Snippet: Antibodies: Antibodies used where: anti-HS (mAb 10E4, AMSBIO, clone F58-10E40), polyclonal goat antiserpinC1/antithrombin III antibody (R&D systems, AF1267), mouse anti-human ATIII (Santa Cruz, clone H-7), anti-Ki67 (ab15580, Abcam), 1:500 anti-CC3 (9661S, Cell Signaling Technology), 1:200 anti-pERK (4370S, Cell Signaling Technology), or 1:200 anti-Vimentin antibodies (5741S, Cell Signaling Technology), α-phospho-Erk1/2 (P-Erk, thr202/tyr204; Cell Signaling, 9101) and α-Erk1/2 (T-Erk, Cell Signaling, 9102), anti-GAPDH [D4C6R] (Cell Signaling Technology, Cat# 971665), Chicken anti-goat Alexa fluor 594 (Invitrogen, #A21468), Donkey anti-Goat IgG, Alexa FluorTM 488 (Invitrogen, Cat# A11055), Donkey anti-goat-HRP (Santa cruz, Sc-2020), Goat-anti-mouse IgG-HRP (Biorad Labs, Cat#170-6516), Goat anti Mouse IgG, Alexa Fluor 488 (Invitrogen, A11001), Goat anti-mouse IgM, Alexa fluor 488 (Invitrogen, A21042).



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